Composite

Part:BBa_K1423005:Design

Designed by: Shawna Henry   Group: iGEM14_WPI-Worcester   (2014-09-23)


Const. Promoter-RBS-BclA-YFP-DT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During the design of this multiple domain sequence, we needed to ensure that all domains remained in the same reading frame.


Source

BclA is from bacillus anthracis while YFP is a mutant GFP from the jellyfish Aequorea victoria.

References